BIRC2 ELISA Kit (Human) (OKEH02117)

Catalog No: OKEH02117

Size:96T

Human BIRC2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology BIRC2 ELISA Kit (Human) (OKEH02117) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BIRC2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BIRC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BIRC2 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.9% (n = 20)
  Mean Inter-assay CV%: < 8.4% (n = 20)
• ELISA Kit Component:
  • BIRC2 Microplate: 96 Wells (12 x 8 Well strips)
  • BIRC2 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated BIRC2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 34 pg/mL
• Specificity: Natural and recombinant Human Baculoviral IAP repeat-containing protein 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BIRC2
• Gene Full Name: baculoviral IAP repeat containing 2
• Alias Symbols: API1;apoptosis inhibitor 1;baculoviral IAP repeat-containing protein 2;cellular inhibitor of apoptosis 1;cIAP1;c-IAP1;Hiap-2;HIAP2;IAP homolog B;IAP-2;inhibitor of apoptosis protein 2;MIHB;NFR2-TRAF signalling complex protein;RING finger protein 48;RING-type E3 ubiquitin transferase BIRC2;RNF48;TNFR2-TRAF-signaling complex protein 2.
• NCBI Gene Id: 329
• Protein Name: Q13490
• Description of Target: Baculoviral IAP repeat-containing protein 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q13490
• Protein Accession #: NP_001157.1
• Nucleotide Accession #: NM_001166.4
• Protein Size (# AA): 618
• Molecular Weight: 70 kDa