BIRC2 Antibody (OAAB19890)

Catalog No: OAAB19890

Size:100ul

Anti-cIAP1 Antibodyat 1: 1000 dilution + HT-29 whole cell lysates
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 70 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Application Info: WB: 1:1000
• Reference: Rothe M.,et al.Cell 83:1243-1252(1995).
  Liston P.,et al.Nature 379:349-353(1996).
  Uren A.G.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:4974-4978(1996).
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Taylor T.D.,et al.Nature 440:497-500(2006).
  

Target Info

• Gene Symbol: BIRC2
• Alias Symbols: API1, MIHB, HIAP2, RNF48, cIAP1, Hiap-2, c-IAP1
• NCBI Gene Id: 329
• Description of Target: Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin- protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase- dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle.
• Uniprot ID: Q13490
• Molecular Weight: 70 kDa