Anti-Retinoblastoma Antibody (OAAI00286)

Catalog No: OAAI00286

Size:100UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Monoclonal
• Clone: ABM353
• Isotype: IgG1
• Host: Mouse
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: The retinoblastoma (RB), a tumor suppressor protein, is a negative regulator of the cell cycle. This protein stabilizes constitutive heterochromatin to maintain the overall chromating structure. It can be inactivated by phosphorylation during G1/S stage by one or more cyclin-dependent kinases (CDKs). Different phosphorylation sites of RB may require more than one different CDKs which leads to a differential regulation of downstream effectors’ pathways. In the hypophosphorylated state, pRB is active and binds transcription factor E2F1, carries out its role as a tumor suppressor by inhibiting cell cycle progression. Phosphorylation by CDks inactives pRB which causes childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. pRB is widely expressed in a variety of human tissues including mammary carcinoma, esophageal cancer, squamous cell carcinoma and cervical cancer.
  Positive Control: Prostate Carcinoma
  Cellular Location: Nuclear
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A recombinant protein of human retinoblastoma (Rb).
• Purification: The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
• Concentration: 100ug/200ul
• Specificity: RB1
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested

Target Info

• Gene Symbol: RB1
• Gene Full Name: RB transcriptional corepressor 1
• Alias Symbols: exon 17 tumor GOS561 substitution mutation causes premature stop;GOS563 exon 17 substitution mutation causes premature stop;OSRC;p105-Rb;p110-RB1;pp110;PPP1R130;pRb;prepro-retinoblastoma-associated protein;protein phosphatase 1, regulatory subunit 130;RB;retinoblastoma 1;retinoblastoma suspectibility protein;retinoblastoma-associated protein.
• NCBI Gene Id: 5925
• Uniprot ID: P06400
• Molecular Weight: 110