Anti-RANKL (human) (Ranky-1) Antibody (OAED00365)

Catalog No: OAED00365

Size:100UG

Figure: Immunohistochemical staining of endogenous human RANKL in different human tissues (paraffin sections) using MAb to RANKL (human)(Ranky-1). Method: Different human tissues (A: Duodenum; B: Small intestine) were stained with MAb to RANKL (human) (Ranky-1) by standard immunohistochemistry. Pictures courtesy of Human Protein Atlas (www.proteinatlas.org).

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: Ranky-1
• Isotype: IgG1
• Host: Rat
• Application: Enzyme-linked immunosorbent assay|Western blot
• Reconstitution and Storage: Long Term Storage : -20C
  Short Term Storage : +4C
  Handling : Avoid freeze/thaw cycles.
  Stable for at least 1 year after receipt when stored at -20C.
• Immunogen: Recombinant human soluble RANKL extracellular domain (aa 151-316) (Prod. No. ALX-522-012).
• Concentration: 1mg/ml
• Specificity: Recognizes human RANKL.
• Source: Purified from concentrated hybridoma tissue culture supernatant.
• Formulation: Liquid. In PBS containing 0.02% sodium azide.
• Purity: ≥95% (SDS-PAGE)
• Application Info: ELISA (capture)
  Immunohistochemistry (paraffin sections)
  Western Blot (1:1,000)
  Note: Does not work for Flow Cytometry or Immunoprecipitation. Does not block RANK-RANKL interaction.
• Reference: The molecular triad OPG/RANK/RANKL: involvement in the orchestration of pathophysiological bone remodeling: S. Theoleyre, et al.; Cytokine Growth Factor Rev. 15, 457 (2004), Abstract;

Target Info

• Gene Symbol: TNFSF11
• Gene Full Name: TNF superfamily member 11
• Alias Symbols: CD254;hRANKL2;ODF;OPGL;OPTB2;osteoclast differentiation factor;osteoprotegerin ligand;RANKL;receptor activator of nuclear factor kappa B ligand;Receptor activator of nuclear factor kappa-B ligand;sOdf;TNF-related activation-induced cytokine;TNLG6B;TRANCE;tumor necrosis factor (ligand) superfamily, member 11;tumor necrosis factor ligand 6B;tumor necrosis factor ligand superfamily member 11;tumor necrosis factor superfamily member 11.
• NCBI Gene Id: 8600
• Protein Name: Tumor necrosis factor ligand superfamily member 11
• Description of Target: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).
• Uniprot ID: O14788