Catalog No: OKCA00201
Size:96 Wells
Price: $550.00
SKU
OKCA00201
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
---|
Predicted Species Reactivity | Bovine | ||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology ANGPT1 ELISA Kit (Bovine) (OKCA00201) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bovine ANGPT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bovine ANGPT1 is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Bovine ANGPT1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 12.5 ng/mL - 800 ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay: CV% < 8% (n = 20). Inter-assay: CV% < 10% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates | ||||||||||||||||||||||
Sensitivity | < 1.384 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of bovine ANGPT1. No significant cross-reactivity or interference between bovine ANGPT1 and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | ANGPT1 |
---|---|
Gene Full Name | angiopoietin 1 |
Alias Symbols | ANG1, ANG-1, Angiopoietin-1 |
NCBI Gene Id | 282140 |
Protein Name | Angiopoietin-1 |
Description of Target | Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This protein is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. |
Uniprot ID | O18920 |
Protein Accession # | NP_001070265 |
Nucleotide Accession # | NM_001076797 |
Protein Size (# AA) | 497 |
Molecular Weight | 57 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review
We found other products you might like!