ANGPT1 ELISA Kit (Bovine) : 96 Wells (OKCA00201)

Catalog No: OKCA00201

Size:96 Wells

Product Info

• Predicted Species Reactivity: Bovine
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva Systems Biology ANGPT1 ELISA Kit (Bovine) (OKCA00201) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bovine ANGPT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bovine ANGPT1 is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Bovine ANGPT1 captured in well.
• ELISA Kit Range: 12.5 ng/mL - 800 ng/mL
• ELISA Kit Reproducibility: Intra-assay: CV% < 8% (n = 20). Inter-assay: CV% < 10% (n = 20)
• ELISA Kit Component:
  • Anti-ANGPT1 Microplate: 96 Well (12 x 8 Well Strips)
  • ANGPT1 Standard (Lyophilized): 2 x 800 ng
  • 100X ANGPT1 Detection Antibody: 1 x 120 uL
  • 100X HRP-Avidin: 1 x 120 uL
  • Detection Antibody Diluent: 1 x 15 mL
  • HRP-Avidin Diluent: 1 x 15 mL
  • Sample Diluent: 1 x 50 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: < 1.384 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of bovine ANGPT1. No significant cross-reactivity or interference between bovine ANGPT1 and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ANGPT1
• Gene Full Name: angiopoietin 1
• Alias Symbols: ANG1, ANG-1, Angiopoietin-1
• NCBI Gene Id: 282140
• Protein Name: Angiopoietin-1
• Description of Target: Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This protein is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
• Uniprot ID: O18920
• Protein Accession #: NP_001070265
• Nucleotide Accession #: NM_001076797
• Protein Size (# AA): 497
• Molecular Weight: 57 kDa