ADCY5 ELISA Kit (Human) (OKCD00835)

Catalog No: OKCD00835 (Formerly GWB-KBBVO8)

Size:96WELLS

Human ADCY5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 5 (ADCY5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 5 (ADCY5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 5 (ADCY5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adenylate Cyclase 5 (ADCY5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase 5 (ADCY5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase 5 (ADCY5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ADCY5 Microplate: 96 Wells (12 x 8 Well Strips)
  • ADCY5 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated ADCY5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.124 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase 5 (ADCY5). No significant cross-reactivity or interference between Adenylate Cyclase 5 (ADCY5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ADCY5
• Gene Full Name: adenylate cyclase 5
• Alias Symbols: AC5;adenylate cyclase type 5;adenylate cyclase type V;adenylyl cyclase 5;ATP pyrophosphate-lyase 5;epididymis secretory sperm binding protein;FDFM.
• NCBI Gene Id: 111
• Protein Name: Adenylate cyclase type 5
• Description of Target: Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling (PubMed:15385642, PubMed:26206488, PubMed:24700542). Mediates signaling downstream of ADRB1 (PubMed:24700542). Regulates the increase of free cytosolic Ca(2+) in response to increased blood glucose levels and contributes to the regulation of Ca(2+)-dependent insulin secretion (PubMed:24740569).
• Uniprot ID: O95622
• Protein Accession #: NP_001186571
• Nucleotide Accession #: NM_001199642.1
• Protein Size (# AA): 1261