IL22RA2 ELISA Kit (Human) (OKCD01834)

Catalog No: OKCD01834 (Formerly GWB-KBBFS4)

Size:96WELLS

Human IL22RA2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	78-103%	79-91%	80-103%	86-93%
  EDTA Plasma (n=5)	80-92%	92-105%	80-103%	93-103%
  Heparin Plasma (n=5)	94-101%	82-101%	87-94%	80-93%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 22 Receptor Alpha 2 (IL22Ra2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 22 Receptor Alpha 2 (IL22Ra2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 22 Receptor Alpha 2 (IL22Ra2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 22 Receptor Alpha 2 (IL22Ra2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	78-95	91
  EDTA Plasma (n=5)	97-105	102
  Heparin Plasma (n=5)	84-93	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 22 Receptor Alpha 2 (IL22Ra2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 22 Receptor Alpha 2 (IL22Ra2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IL22RA2 Microplate: 96 Wells (12 x 8 Well Strips)
  • IL22RA2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated IL22RA2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.129 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 22 Receptor Alpha 2 (IL22Ra2). No significant cross-reactivity or interference between Interleukin 22 Receptor Alpha 2 (IL22Ra2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IL22RA2
• Gene Full Name: interleukin 22 receptor subunit alpha 2
• Alias Symbols: CRF2-10;CRF2-S1;CRF2X;cytokine receptor class-II member 10;Cytokine receptor family 2 member 10;cytokine receptor family type 2, soluble 1;IL-22BP;IL-22RA2;IL-22R-alpha-2;interleukin 22 receptor, alpha 2;interleukin 22-binding protein;interleukin-22 receptor subunit alpha-2;Interleukin-22-binding protein;ZCYTOR16.
• NCBI Gene Id: 116379
• Protein Name: Interleukin-22 receptor subunit alpha-2
• Description of Target: Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.
• Uniprot ID: Q969J5
• Protein Accession #: NP_443194.1
• Nucleotide Accession #: NM_052962.2
• Protein Size (# AA): 263