IL22RA2 ELISA Kit (Human) (OKCA02113)

Catalog No: OKCA02113

Size:96 Wells

Human IL22RA2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	94	87-98
  Serum (n=4)	1:2	107	103-112
  Serum (n=4)	1:4	91	85-97
  Serum (n=4)	1:8	92	88-96

• ELISA Kit Principle: Aviva Systems Biology IL22RA2 ELISA Kit (Human) (OKCA02113) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL22RA2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL22RA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL22RA2 captured in well.
• ELISA Kit Range: 31.25 pg/mL-2,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	102	98-105
  EDTA plasma (n=4)	98	93-104

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • IL22RA2 Microplate: 96 Wells (12 x 8 Well strips)
  • IL22RA2 Lyophilized Standard: 2 x 3 ng
  • 100X Biotinylated IL22RA2 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • (???)X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 7.81 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL22RA2
• Alias Symbols: CRF2X, CRF2-10, CRF2-S1, IL-22BP, IL-22RA2, ZCYTOR16, IL-22R-alpha-2
• NCBI Gene Id: 116379
• Protein Name: Interleukin-22 receptor subunit alpha-2
• Description of Target: Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses. Isoform 1 may play a role in establishing and maintaining successful pregnancy.
• Uniprot ID: Q969J5
• Protein Accession #: NP_443194
• Nucleotide Accession #: NM_052962