TUBA1A Antibody (OAGA00798)

Catalog No: OAGA00798

Size:100 ul

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
10% SDS PAGE
OAGA00798 diluted at 1:10000

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 ?g drosophila lysate/extract
10 % SDS-PAGE
alpha Tubulin 1A antibody (OAGA00798) dilution: 1:1000

Sample (30 ug of whole cell lysate)
A: HeLa
B: Hep G2
C: Molt-4
10% SDS PAGE
OAGA00798 diluted at 1:10000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
OAGA00798 diluted at 1:10000

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 50 ?g rat brain lysate/extract
10 % SDS-PAGE
alpha Tubulin 1A antibody (OAGA00798) dilution: 1:10000

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using alpha Tubulin 1A(OAGA00798) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha 1A(OAGA00798) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Drosophila
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. [provided by RefSeq]
  Positive Controls: 293T, NIH-3T3, HeLa, Hep G2, Molt-4, rat brain, drosophila, *mouse testis
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 37 and 276 of alpha Tubulin 1A
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.91 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: TUBA1A
• Gene Full Name: Tubulin alpha-1A chain
• Alias Symbols: LIS3, TUBA3, B-ALPHA-1
• NCBI Gene Id: 7846
• Protein Name: Tubulin alpha-1A chain
• Uniprot ID: Q71U36
• Protein Accession #: NP_001257328.1
• Molecular Weight: 50 kDa