TRAF5 ELISA Kit (Human) (OKCD00514)

Catalog No: OKCD00514 (Formerly GWB-KBBON1)

Size:96WELLS

Human TRAF5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	85-99%	99-105%	93-101%	82-101%
  EDTA Plasma (n=5)	89-99%	84-91%	84-98%	80-96%
  Heparin Plasma (n=5)	78-89%	94-101%	88-102%	96-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF Receptor Associated Factor 5 (TRAF5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNF Receptor Associated Factor 5 (TRAF5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF Receptor Associated Factor 5 (TRAF5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNF Receptor Associated Factor 5 (TRAF5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	78-91	81
  EDTA plasma(n=5)	95-103	99
  heparin plasma(n=5)	84-93	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNF Receptor Associated Factor 5 (TRAF5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNF Receptor Associated Factor 5 (TRAF5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TRAF5 Microplate: 96 Wells (12 x 8 Well Strips)
  • TRAF5 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated TRAF5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.064 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of TNF Receptor Associated Factor 5 (TRAF5). No significant cross-reactivity or interference between TNF Receptor Associated Factor 5 (TRAF5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TRAF5
• Gene Full Name: TNF receptor associated factor 5
• Alias Symbols: MGC:39780;RING finger protein 84;RNF84;TNF receptor-associated factor 5.
• NCBI Gene Id: 7188
• Protein Name: TNF receptor-associated factor 5
• Description of Target: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR.
• Uniprot ID: O00463
• Protein Accession #: NP_001029082
• Nucleotide Accession #: NM_001033910.2
• Protein Size (# AA): 557