TPD52 ELISA Kit (Human) (OKEH02283)

Catalog No: OKEH02283

Size:96T

Human TPD52 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology TPD52 ELISA Kit (Human) (OKEH02283) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human TPD52 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TPD52 and incubated. The TPD52 found in the sample or standards competes with the biotinylated TPD52 for limited binding sites on the immobilized anti-Human TPD52 antibody. Excess unbound biotinylated TPD52 and sample or standard TPD52 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TPD52 captured in the well and inversely proportional to the amount of TPD52 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.5% (n = 20)
  Mean Inter-assay CV%: < 8.6% (n = 20)
• ELISA Kit Component:
  • THOP1 Microplate: 96 Wells (12 x 8 Well strips)
  • THOP1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated THOP1 Detector Antibody: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.2 ng/mL
• Specificity: Natural and recombinant Human Tumor protein D52
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: TPD52
• Gene Full Name: tumor protein D52
• Alias Symbols: D52;hD52;N8L;PC-1;PrLZ;prostate and colon associated protein;prostate leucine zipper;protein N8;tumor protein D52.
• NCBI Gene Id: 7163
• Protein Name: P55327
• Description of Target: Tumor protein D52
• Uniprot ID: https://www.uniprot.org/uniprot/P55327
• Protein Accession #: NP_001020423.1
• Nucleotide Accession #: NM_001025252.2
• Protein Size (# AA): 224
• Molecular Weight: 24 kDa