Thrombopoietin Receptor Auto-Antibody (IgG) (Human) ELISA Kit (OKCA00442)

Catalog No: OKCA00442

Size:96 Wells

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology Thrombopoietin Receptor Auto-Antibody (IgG) (Human) ELISA Kit (OKCA00442) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Thrombopoietin Receptor antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanIgG is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Thrombopoietin Receptor Auto-Antibody (IgG) captured in well.
• ELISA Kit Reproducibility: Three different samples of known Thrombopoietin Receptor Auto-Antibody (IgG) concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each.
  Intra-Assay CV: <15%
  Inter-Assay CV: <15%
• ELISA Kit Component:
  • Thrombopoietin Receptor Auto-Antibody (IgG) Microplate: 96 Wells (12 x 8 Well strips)
  • Negative Control: 1 x 800 ul
  • Positive Control: 1 x 800 ul
  • Anti-Human IgG HRP-conjugate: 1 x 10 ml
  • Sample Diluent: 1 x 20 ml
  • 25X Wash Buffer: 1 x 20 ml
  • Substrate A: 1 x 5 ml
  • Substrate B: 1 x 5 ml
  • Stop Solution: 1 x 5 ml
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum
• Assay Info: Assay Methodology: Qualitative Reverse Capture ELISA

Target Info

• Alias Symbols: C-MPL
• Protein Name: Thrombopoietin Receptor Auto-Antibody (IgG)
• Description of Target: Thrombopoietin Receptor Auto-Antibody (IgG)