TFF3 ELISA Kit (Human) (OKBB00556)

Catalog No: OKBB00556

Size:96 Wells

Human TFF3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human TFF3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFF3 has been precoated onto 96-well plates. Standards(E.coli, E22-Y80) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for TFF3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human TFF3 amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	164	425	631	152	395	640
  SD	7.38	22.1	25.87	9.6	28	37.76
  CV%	4.5	5.2	4.1	6.3	7.1	5.9

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human TFF3 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human TFF3 Standard: 2 x 1 ng/tube
  • 100X Biotinylated Anti-Human TFF3 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human TFF3
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: E22-Y80
• Description: For quantitative detection of human TFF3 in cell culture supernatants, serum, plasma(heparin, EDTA), saliva and urine.

Target Info

• Gene Symbol: TFF3
• Gene Full Name: trefoil factor 3 (intestinal)
• Alias Symbols: hITF, hP1.B, Intestinal trefoil factor, ITF, mITF, OTTMUSP00000021729, Polypeptide P1.B, TFF3, TFF3_HUMAN, TFI, TREFOIL, Trefoil factor (intestinal), Trefoil factor 3
• NCBI Gene Id: 7033
• Protein Name: Trefoil factor 3
• Description of Target: Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.
• Uniprot ID: Q07654
• Protein Accession #: NP_003217.3
• Nucleotide Accession #: NM_003226.3