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Datasheets/Manuals | Printable datasheet for STAT5 Polyclonal Antibody (OABB00791) |
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Tested Species Reactivity | Human, Mouse, Rat |
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Predicted Species Reactivity | Bovine|Human|Mouse|Rat |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Application | Flow cytometry|Western blot |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: STAT5(Signal transducer and activator of transcription 5) also known as STAT5A, MGF, is a protein that serve the dual function of signal transducers and activators of transcription in cells exposed to signaling polypeptides. Hou et al.(1995) cloned the human STAT5 cDNA from an umbilical vein endothelial cell library and found that it encodes a 794-amino acid polypeptide with a predicted mass of approximately 90.5 kD. To analyze the possible role of STAT5 in human GH-induced proliferation, Friedrichsen et al.(2001) expressed a dominant-negative STAT5 mutant, STAT5A-delta-749, in INS-1 cells under the control of a doxycycline-inducible promoter by stable transfection. Chromatin immunoprecipitation analysis confirmed direct binding of STAT5 to the HIF2A promoter 344 bp upstream of the HIF2A start site. STAT5 overexpression independently resulted in erythroid commitment in megakaryocytic-erythroid progenitors, which was abrogated by knockdown of GATA1. STAT5 is responsible for the immediate-early induction of the long isoform of the BCL2-related protein in erythroid cells through direct binding to the promoter of the BCLX gene. |
:: | Background: STAT5(Signal transducer and activator of transcription 5) also known as STAT5A, MGF, is a protein that serve the dual function of signal transducers and activators of transcription in cells exposed to signaling polypeptides. Hou et al. (1995) cloned the human STAT5 cDNA from an umbilical vein endothelial cell library and found that it encodes a 794-amino acid polypeptide with a predicted mass of approximately 90.5 kD. To analyze the possible role of STAT5 in human GH-induced proliferation, Friedrichsen et al. (2001) expressed a dominant-negative STAT5 mutant, STAT5A-delta-749, in INS-1 cells under the control of a doxycycline-inducible promoter by stable transfection. Chromatin immunoprecipitation analysis confirmed direct binding of STAT5 to the HIF2A promoter 344 bp upstream of the HIF2A start site. STAT5 overexpression independently resulted in erythroid commitment in megakaryocytic-erythroid progenitors, which was abrogated by knockdown of GATA1. STAT5 is responsible for the immediate-early induction of the long isoform of the BCL2-related protein in erythroid cells through direct binding to the promoter of the BCLX gene |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Polypeptide |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat Flow Cytometry: 1-3 ug/1x10^6 cells: Human |
Reference | 1. Friedrichsen, B. N., Galsgaard, E. D., Nielsen, J. H., Moldrup, A. Growth hormone- and prolactin-induced proliferation of insulinoma cells, INS-1, depends on activation of STAT5 (signal transducer and activator of transcription 5). Molec. Endocr. 15: 136-148, 2001. 2. Hou, J., Schindler, U., Henzel, W. J., Wong, S. C., McKnight, S. L. Identification and purification of human Stat proteins activated in response to interleukin-2. Immunity 2: 321-329, 1995. 3. Schwaller, J., Parganas, E., Wang, D., Cain, D., Aster, J. C., Williams, I. R., Lee, C.-K., Gerthner, R., Kitamura, T., Frantsve, J., Anastasiadou, E., Loh, M. L., Levy, D. E., Ihle, J. N., Gilliland, D. G. Stat5 is essential for the myelo- and lymphoproliferative disease induced by TEL/JAK2.Molec. Cell 6: 693-704, 2000 |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 5A(STAT5A) detection. Tested with WB in Human. |
Gene Symbol | STAT5A |
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Gene Full Name | signal transducer and activator of transcription 5A |
Alias Symbols | epididymis secretory sperm binding protein;MGF;signal transducer and activator of transcription 5A;STAT5. |
NCBI Gene Id | 6776 |
Protein Name | Signal transducer and activator of transcription 5A |
Description of Target | Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. |
Uniprot ID | P42229 |
Molecular Weight | 90647 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "STAT5 Polyclonal Antibody (OABB00791)"?
The tested species reactivity for this item is "Human, Mouse, Rat". This antibody is predicted to have homology to "Bovine|Human|Mouse|Rat".
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How long will it take to receive "STAT5 Polyclonal Antibody (OABB00791)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "STAT5 Polyclonal Antibody (OABB00791)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "STAT5 Polyclonal Antibody (OABB00791)"?
This target may also be called "epididymis secretory sperm binding protein;MGF;signal transducer and activator of transcription 5A;STAT5." in publications.
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What is the shipping cost for "STAT5 Polyclonal Antibody (OABB00791)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "STAT5 Polyclonal Antibody (OABB00791)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "STAT5 Polyclonal Antibody (OABB00791)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "90647 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "STAT5 Polyclonal Antibody (OABB00791)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "STAT5A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "STAT5A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "STAT5A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "STAT5A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "STAT5A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "STAT5A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.