Slit2 ELISA Kit (Rat) (OKDD00641)

Catalog No: OKDD00641 (Formerly GWB-KBBOP7)

Size:96 Wells

Rat Slit2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology Slit2 ELISA Kit (Rat) (OKDD00641) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Slit2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slit2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Slit2 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

  Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

  Mean Intra-Assay: CV<10%

  Mean Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-Slit2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Slit2 Lyophilized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X Slit2 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates
• Sensitivity: <0.053 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Slit2. No significant cross-reactivity or interference between Slit2 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Slit2
• Gene Full Name: slit guidance ligand 2
• Alias Symbols: slit homolog 2 protein;slit-2.
• NCBI Gene Id: 360272
• Protein Name: Slit homolog 2 protein
• Description of Target: Acts as a ligand for glypican-1, a heparan sulfate proteoglycan; may play a role in neurogenesis and midline development [rgd, feb 2006]
• Uniprot ID: F1MA79
• Protein Accession #: NP_072154.2
• Nucleotide Accession #: NM_022632.2
• Protein Size (# AA): 1525
• Molecular Weight: 169 kDa