SLC40A1 ELISA Kit (Mouse) (OKEH04277)

Catalog No: OKEH04277

Size:96T

Mouse SLC40A1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Slc40a1 ELISA Kit (Mouse) (OKEH04277) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc40a1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slc40a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc40a1 captured in the well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.9% (n = 20)
  Mean Inter-assay CV%: <= 9.3% (n = 20)
• ELISA Kit Component:
  • Slc40a1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Slc40a1 Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Slc40a1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.18 ng/mL
• Specificity: Natural and recombinant Mouse Solute carrier family 40 member 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Slc40a1
• Gene Full Name: solute carrier family 40 (iron-regulated transporter), member 1
• Alias Symbols: duodenal-specific;Dus;Dusg;ferroportin 1;ferroportin1;Ferroportin-1;FP;Fpn1;IRE;IREG1;iron-regulated transporter 1;metal transporter protein 1;metal transporting protein 1;MTP;MTP1;O;Ol5;Pc;Pcm;polycythaemia;Slc1;Slc11a3;SLC11A3 iron transporter;Slc39a1;solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3;solute carrier family 39 (iron-regulated transporter), member 1;solute carrier family 40 member 1.
• NCBI Gene Id: 53945
• Protein Name: Q9JHI9
• Description of Target: Solute carrier family 40 member 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q9JHI9
• Protein Accession #: NP_058613.2
• Nucleotide Accession #: NM_016917.2
• Protein Size (# AA): 570
• Molecular Weight: 63 kDa