SLC12A3 ELISA Kit (Human) (OKCD00936)

Catalog No: OKCD00936 (Formerly GWB-KBBCH1)

Size:96WELLS

Human SLC12A3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Na-Cl Cotransporter (NCCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Na-Cl Cotransporter (NCCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Na-Cl Cotransporter (NCCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Na-Cl Cotransporter (NCCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Na-Cl Cotransporter (NCCT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Na-Cl Cotransporter (NCCT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SLC12A3 Microplate: 96 Wells (12 x 8 Well Strips)
  • SLC12A3 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated SLC12A3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.115 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Na-Cl Cotransporter (NCCT). No significant cross-reactivity or interference between Na-Cl Cotransporter (NCCT) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SLC12A3
• Gene Full Name: solute carrier family 12 member 3
• Alias Symbols: Na-Cl cotransporter;NaCl electroneutral thiazide-sensitive cotransporter;Na-Cl symporter;NCC;NCCT;solute carrier family 12 (sodium/chloride transporter), member 3;solute carrier family 12 member 3;thiazide-sensitive Na-Cl cotransporter;thiazide-sensitive sodium-chloride cotransporter;TSC.
• NCBI Gene Id: 6559
• Protein Name: Solute carrier family 12 member 3
• Description of Target: Electroneutral sodium and chloride ion cotransporter. In kidney distal convoluted tubules, key mediator of sodium and chloride reabsorption (PubMed:21613606, PubMed:22009145). Receptor for the proinflammatory cytokine IL18. Contributes to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1 (By similarity).
• Uniprot ID: P55017
• Protein Accession #: NP_000330
• Nucleotide Accession #: NM_000339.2
• Protein Size (# AA): 1021