S100A14 ELISA Kit (Human) (OKCD00896)

Catalog No: OKCD00896 (Formerly GWB-KBBBY5)

Size:96WELLS

Human S100A14 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A14 (S100A14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A14 (S100A14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A14 (S100A14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100 Calcium Binding Protein A14 (S100A14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A14 (S100A14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A14 (S100A14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-S100A14 Microplate: 96 Wells (12 x 8 Well Strips)
  • S100A14 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated S100A14 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A14 (S100A14). No significant cross-reactivity or interference between S100 Calcium Binding Protein A14 (S100A14) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: S100A14
• Gene Full Name: S100 calcium binding protein A14
• Alias Symbols: BCMP84;breast cancer membrane protein 84;protein S100-A14;S100 calcium-binding protein A14;S100A15;S114.
• NCBI Gene Id: 57402
• Protein Name: Protein S100-A14
• Description of Target: Modulates P53/TP53 protein levels, and thereby plays a role in the regulation of cell survival and apoptosis. Depending on the context, it can promote cell proliferation or apoptosis. Plays a role in the regulation of cell migration by modulating the levels of MMP2, a matrix protease that is under transcriptional control of P53/TP53. Does not bind calcium.
• Uniprot ID: Q9HCY8
• Protein Accession #: NP_065723
• Nucleotide Accession #: NM_020672.2
• Protein Size (# AA): 104