RNGTT Antibody (OAGA00421)

Catalog No: OAGA00421

Size:100 ul

Sample (30 ug of whole cell lysate)
A: HeLa
B: Hep G2
C: MOLT4
7.5% SDS PAGE
OAGA00421 diluted at 1:1000

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB
• Additional Information: Background: Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
  Positive Controls: 293T, A431, HeLa, HepG2, Molt-4, Raji
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 40 and 286 of HCE (Uniprot ID#O60942)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.54 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: RNGTT
• Gene Full Name: mRNA-capping enzyme
• Alias Symbols: HCE, HCE1, hCAP, CAP1A
• NCBI Gene Id: 8732
• Protein Name: mRNA-capping enzyme
• Uniprot ID: O60942
• Protein Accession #: NP_003791.3
• Molecular Weight: 69 kDa