RGMB Antibody (OAGA00558)

Catalog No: OAGA00558

Size:100 ul

Sample(30 ug whole cell lysate)
A:A431
B:H1299
C:HeLa S3
10% SDS PAGE
OAGA00558 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
OAGA00558 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RGMB(OAGA00558) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: RGMB is a glycosylphosphatidylinositol (GPI)-anchored member of the repulsive guidance molecule family (see RGMA, MIM 607362) and contributes to the patterning of the developing nervous system (Samad et al., 2005 [PubMed 15671031]).[supplied by OMIM]
  Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain
  Cellular Localization: Cell membrane; Lipid-anchor, GPI-anchor
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 178 and 437 of RGMB (Uniprot ID#Q6NW40)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: RGMB
• Gene Full Name: RGM domain family member B
• Alias Symbols: DRAGON
• NCBI Gene Id: 285704
• Protein Name: RGM domain family member B
• Uniprot ID: Q6NW40
• Protein Accession #: NP_001012779.2
• Molecular Weight: 52 kDa