UBTF Antibody - middle region (ARP33480_P050)

Catalog No: ARP33480_P050

WB Suggested Anti-UBTF Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:2500
Positive Control: Hela cell lysateUBTF is supported by BioGPS gene expression data to be expressed in HeLa

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBTF
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Guinea Pig: 79%; Horse: 77%; Human: 100%; Mouse: 93%; Pig: 86%; Rabbit: 93%; Rat: 93%
• Peptide Sequence: Synthetic peptide located within the following region: LESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPV
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-UBTF (ARP33480_P050) antibody is Catalog # AAP33480 (Previous Catalog # AAPP04530)
• Sample Type Confirmation:

  UBTF is supported by BioGPS gene expression data to be expressed in HeLa

• Reference: Prieto,J.L. (2007) Genes Dev. 21 (16), 2041-2054

Target Info

• Gene Symbol: UBTF
• Gene Full Name: Upstream binding transcription factor, RNA polymerase I
• Alias Symbols: UBF, UBF1, UBF2, UBF-1, CONDBA, NOR-90
• NCBI Gene Id: 7343
• Protein Name: Nucleolar transcription factor 1
• Description of Target: UBTF is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1. Two UBTF polypeptides, of 94 and 97 kD, exist in the human. UBTF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxesUpstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: P17480
• Protein Accession #: NP_055048
• Nucleotide Accession #: NM_014233
• Protein Size (# AA): 764
• Molecular Weight: 89kDa
• Protein Interactions: UBC; MDM2; RNF2; Dlg4; ESR1; CDK4; CDK2; CCNE1; CCND1; WDR70; YEATS4; RB1; GRK5; KAT5; SIRT7; POLR1A; MYBBP1A; HDAC1; RUNX2; SUMO2; SMARCA5; MAPK3; MAPK1; SUMO1; PIK3CA; IRS1; TAF1D; tat; MCRS1; TLE1; ACTN4; POLR1E; CHGB; SMURF1; TCOF1; MAD1L1; ZMYM2; SER