Catalog No: OKEH03329
Size:96T
Price: $600.00
SKU
OKEH03329
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology REC8 ELISA Kit (Human) (OKEH03329) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human REC8 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated REC8 and incubated. The REC8 found in the sample or standards competes with the biotinylated REC8 for limited binding sites on the immobilized anti-Human REC8 antibody. Excess unbound biotinylated REC8 and sample or standard REC8 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated REC8 captured in the well and inversely proportional to the amount of REC8 which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.0% (n = 20)
Mean Inter-assay CV%: <= 7.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-REC8 Microplate96 Wells (12 x 8 Well strips)
REC8 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X REC8-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.39 ng/mL
SpecificityNatural and recombinant Human Meiotic recombination protein REC8 homolog
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolREC8
Gene Full NameREC8 meiotic recombination protein
Alias Symbolscohesin Rec8p;cohesion rec8p;HR21spB;human homolog of rad21, S. pombe;kleisin-alpha;meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family;meiotic recombination protein REC8 homolog;meiotic recombination protein REC8-like 1;REC8 homolog;REC8L1;REC8-like 1;Rec8p;recombination and sister chromatid cohesion protein homolog.
NCBI Gene Id9985
Protein NameO95072
Description of TargetMeiotic recombination protein REC8 homolog
Uniprot IDhttps://www.uniprot.org/uniprot/O95072
Protein Accession #NP_001041670.1
Nucleotide Accession #NM_001048205.1
Protein Size (# AA)547
Molecular Weight63 kDa
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