RAP80 Antibody (OAPB00570)

Catalog No: OAPB00570

Size:100UG

Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 μg/mL.

Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 μg/mL.

Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 μg/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Porcine|Rat
• Product Format: Liquid. RAP80 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: RAP80 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: RAP80 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human RAP80. The immunogen is located within amino acids 30 - 80 of RAP80.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-RAP80 antibody is Catalog # OEPB02680
• Application Info: RAP80 antibody can be used for detection of RAP80 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Yan Z, Kim YS, and Jetten AM. RAP80, a novel nuclear protein that interacts with the retinoid-related testis-associated receptor. J. Biol. Chem.2002; 277:32379-88.;Wang B, Matsuoka S, Balliff BA, et al. Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science2007; 316:1194-1198.;Kim H, Huang J, and Chen J. CCDC98 is a BRCA1-BRCT domain-binding protein involved in the DNA damage response. Nat. Struct. Mol. Biol.2007; 14:710-5.;Liu Z, Wu J, and Yu X. CCDC98 targets BRCA1 to DNA damage sites. Nat. Struct. Mol. Biol.2007; 14:716-20.

Target Info

• Gene Symbol: UIMC1
• Gene Full Name: ubiquitin interaction motif containing 1
• Alias Symbols: BRCA1-A complex subunit RAP80;RAP80;receptor-associated protein 80;retinoid X receptor-interacting protein 110;Ubiquitin interaction motif-containing protein 1;X2HRIP110.
• NCBI Gene Id: 51720
• Protein Name: BRCA1-A complex subunit RAP80|receptor associated protein 80, isoform CRA_c [Homo sapiens]
• Description of Target: Ubiquitin-binding protein (PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.37). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
• Uniprot ID: Q96RL1
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/EAW85043