Biotinylated CCL3 Protein (OPCB00018)

Catalog No: OPCB00018

Size:2ug

Migration Assay: Cells expressing recombinant CCR5 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated MIP-1 alpha. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

Product Info

• Product Format: Lyophilized
• Conjugation: Biotin
• Application: CA
  Endotoxin Level: <0.01 EU per 1ug of the protein by the LAL method
  Extinction Coefficient: 15,460 M-1 cm-1
• Reconstitution and Storage: Reconstitution: Spin tube prior to resuspending. Recommended at 100 ug/mL in sterile water. Use immediately after reconstitution.
  Store at -20°C to -70°C for at least 1 month under sterile conditions after reconstitution. Stable for 12 months from date of receipt, stored at -20°C to -70°C.
• Purity: > 97% as determined by SDS-PAGE
• Biological Activity: EC50 = 1.1-2.6nM determined by Migration Assay of recombinant CCR5 containing cells
  
• Source: E. coli
• Reference: 1. "Macrophage inflammatory protein-1"
  Menten P., Wuyts A., Van Damme J.
  Cytokine Growth Factor Rev. 13:455-481(2002)
  2. "Identification of RANTES, MIP-1, and MIP-1[1] as the major HIV-suppressive factors produced by CD8+T cells"
  Cocci F., De Vico A.L., Garzino-Demo A., Arya S.K., Gallo R.C., Lusso P.
  Science 270:1811-1815 (1995)
  3. "Identification and characterization of an inhibitor of haemopoietic stem cell proliferation"
  Graham G.J., Wright E.G., Hewick R., Wolpe S.D., Wilkie N.M., Donaldson D., Lorimore S., Pragnell I.B.
  Nature 344:442-444 (1990)
  4. "Mitogenic activation of human T cells induces two closely related genes which share structural similarities with a new family of secreted factors"
  Zipfel P.F., Balke J., Irving S.G., Kelly K., Siebenlist U.
  J Immunol 142:1582-1590 (1989)
  

Target Info

• Gene Symbol: CCL3
• Gene Full Name: chemokine (C-C motif) ligand 3
• Alias Symbols: MIP1A, SCYA3, G0S19-1, LD78ALPHA, MIP-1-alpha
• NCBI Gene Id: 6348
• Description of Target: Macrophage inflammatory proteins 1-alpha (MIP1alpha)(CCL3) binds to cell surface receptors CCR1 and CCR5. It is proinflammatory, leading to chemotaxis and activation of immune cells. It also inhibits proliferation of hematopoietic stem cells. Moreover, by binding to one of the HIV coreceptors, CCR5, it suppresses HIV infection.
• Uniprot ID: P10147
• Molecular Weight: 10 kDa