Catalog No: OKBB00475
Size:96 Wells
Price: $583.00
SKU
OKBB00475
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse Edn1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Edn1 has been precoated onto 96-well plates. Standards (mouse peptide,C53-W73) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Edn1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Edn1 amount of sample captured in plate.
ELISA Kit Range3.9 pg/ml - 250 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)22.285.714421.491.5153
SD0.7774.118.211.466.6812.09
CV%3.54.85.76.87.37.9
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse Endothelin Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse Endothelin Standard2 x 1 ng/tube
100X Biotinylated Anti-Mouse Endothelin Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<0.5 pg/ml
SpecificityNatural and recombinant mouse Endothelin
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: human peptide; Immunogen sequence: C53-W73
DescriptionFor quantitative detection of mouse Edn1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolEDN1
Gene Full Nameendothelin 1
Alias SymbolsBig endothelin 1, ET1, HDLCQ7, PPET1, Preproendothelin 1, EDN2, EDN2_HUMAN, Endothelin 2, Endothelin-2, ET 2, ET-2, ET2, PPET2, Preproendothelin 2, Preproendothelin-2, EDN3, EDN3_HUMAN, Endothelin-3, ET 3, ET-3, PPET3, Preproendothelin 3, Preproendothelin-3
NCBI Gene Id13614
Protein NameEndothelin-1
Description of TargetThe endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.
Uniprot IDP22387
Protein Accession #NP_034234.1
Nucleotide Accession #NM_010104.3
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