PRDX4 ELISA Kit (Mouse) (OKCD02820)

Catalog No: OKCD02820

Size:96WELLS

Mouse PRDX4 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	81-96%	78-94%	81-104%	84-95%
  EDTA Plasma (n=5)	78-91%	83-96%	94-101%	92-99%
  Heparin Plasma (n=5)	95-103%	83-91%	84-94%	93-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 4 (PRDX4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxiredoxin 4 (PRDX4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxiredoxin 4 (PRDX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peroxiredoxin 4 (PRDX4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	81-95	84
  EDTA Plasma (n=5)	82-101	94
  Heparin Plasma(n=5)	91-101	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxiredoxin 4 (PRDX4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxiredoxin 4 (PRDX4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PRDX4 Microplate: 96 Wells (12 x 8 Well strips)
  • PRDX4 Lyophilized Standard: 2
  • 100X Biotinylated PRDX4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 12.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peroxiredoxin 4 (PRDX4). No significant cross-reactivity or interference between Peroxiredoxin 4 (PRDX4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Prdx4
• Gene Full Name: peroxiredoxin 4
• Alias Symbols: antioxidant enzyme AOE372;AOE3;AOE372;peroxiredoxin IV;peroxiredoxin-4;Pr;Prx IV;Prx4;Prx-iv;thioredoxin peroxidase AO372;thioredoxin-dependent peroxide reductase A0372;thioredoxin-dependent peroxiredoxin 4;TRANK.
• NCBI Gene Id: 53381
• Protein Name: Peroxiredoxin-4
• Description of Target: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events (PubMed:11229364). Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation (By similarity).
• Uniprot ID: O08807
• Protein Accession #: NP_001300640.1
• Nucleotide Accession #: NM_001313711.1
• Protein Size (# AA): 274