PRDX1 ELISA Kit (Rat) (OKCD02786)

Catalog No: OKCD02786

Size:96WELLS

Rat PRDX1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	83-105%	80-90%	80-102%	96-103%
  EDTA Plasma (n=5)	80-102%	87-96%	81-102%	81-104%
  Heparin Plasma (n=5)	83-94%	98-105%	82-103%	85-92%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 1 (PRDX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxiredoxin 1 (PRDX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxiredoxin 1 (PRDX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peroxiredoxin 1 (PRDX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90-101	95
  EDTA Plasma (n=5)	89-101	95
  Heparin Plasma(n=5)	79-95	82

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxiredoxin 1 (PRDX1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxiredoxin 1 (PRDX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PRDX1 Microplate: 96 Wells (12 x 8 Well strips)
  • PRDX1 Lyophilized Standard: 2
  • 100X Biotinylated PRDX1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.063 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peroxiredoxin 1 (PRDX1). No significant cross-reactivity or interference between Peroxiredoxin 1 (PRDX1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Prdx1
• Gene Full Name: peroxiredoxin 1
• Alias Symbols: Hbp23;heme-binding 23 kDa protein;peroxiredoxin-1;thioredoxin peroxidase 2;thioredoxin-dependent peroxide reductase 2;thioredoxin-dependent peroxiredoxin 1.
• NCBI Gene Id: 117254
• Protein Name: Peroxiredoxin-1
• Description of Target: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (By similarity). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).
• Uniprot ID: Q63716
• Protein Accession #: NP_476455.1
• Nucleotide Accession #: NM_057114.1
• Protein Size (# AA): 199