PRDM14 Antibody - N-terminal region (OAAB08391)

Catalog No: OAAB08391

Size:400ul

Western blot analysis of PRDM14 polyclonal antibody (Cat. #OAAB08391) in A549 cell lysate. PRDM14 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

IFanalysis of PRDM14 in breast cancer cells. SKBr-3 cells were transfected with pCMV-Tag2A or pCMV-PRDM14 and then labeled with anti-PRDM14 antibody. The nucleus was stained with DAPI.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, IF
• Additional Information: Precautions: PRDM14 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression (By similarity) Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'
  Cellular Location: Nucleus.
  Tissue Location: Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PRDM14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PRDM14.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  IF: 1:10~50

Target Info

• Gene Symbol: PRDM14
• Alias Symbols: PFM11
• NCBI Gene Id: 63978
• Protein Name: PR domain zinc finger protein 14
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9GZV8
• Protein Accession #: NP_078780.1
• Molecular Weight: 64062 Da