POLR2H Antibody - N-terminal region (ARP48697_T100)

Catalog No: ARP48697_T100

WB Suggested Anti-POLR2H Antibody Titration: 5.0ug/ml
Positive Control: HepG2 cell lysatePOLR2H is supported by BioGPS gene expression data to be expressed in HepG2

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POLR2H
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: DLGDKFRLVIASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIE
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-POLR2H (ARP48697_T100) antibody is Catalog # AAP48697 (Previous Catalog # AAPP28747)
• Sample Type Confirmation:

  POLR2H is supported by BioGPS gene expression data to be expressed in HepG2

• Subunit: RPABC3
• Reference: Ewing,R.M., Mol. Syst. Biol. 3, 89 (2007)

Target Info

• Gene Symbol: POLR2H
• Gene Full Name: Polymerase (RNA) II (DNA directed) polypeptide H
• Alias Symbols: RPB8, RPB17, RPABC3
• NCBI Gene Id: 5437
• Protein Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3
• Description of Target: POLR2H is one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III.This gene encodes one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-400 AU142999.1 1-400 401-907 BI772069.1 287-793 908-1792 BC008348.1 928-1812 1793-3185 AC099344.4 111461-112853 c
• Uniprot ID: P52434
• Protein Accession #: NP_006223
• Nucleotide Accession #: NM_006232
• Protein Size (# AA): 150
• Molecular Weight: 17kDa
• Protein Interactions: TCEB3; SRC; PSMB9; ESR1; CEP57; RNF2; SNRNP200; SART3; POLR2E; DNMT3B; DNMT3A; BAG3; POLR2C; MED19; METTL18; MED26; UBC; POLR3D; POLR3H; POLR3B; POLR1A; POLR3A; POLR1C; NEDD8; Atf7ip; SUMO2; INTS10; POLR2D; POLR2A; BRCA1; BARD1; INTS5; INTS3; INTS6; INTS1