NT5C3I ELISA Kit (Rat) (OKEH02412)

Catalog No: OKEH02412

Size:96T

Rat NT5C3I ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology NT5C3I ELISA Kit (Rat) (OKEH02412) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat NT5C3I antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated NT5C3I and incubated. The NT5C3I found in the sample or standards competes with the biotinylated NT5C3I for limited binding sites on the immobilized anti-Rat NT5C3I antibody. Excess unbound biotinylated NT5C3I and sample or standard NT5C3I is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated NT5C3I captured in the well and inversely proportional to the amount of NT5C3I which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.1% (n = 20)
  Mean Inter-assay CV%: < 7.5% (n = 20)
• ELISA Kit Component:
  • Anti-NT5C3I Microplate: 96 Wells (12 x 8 Well strips)
  • NT5C3I Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X NT5C3I Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.182 ng/mL
• Specificity: Natural and recombinant Rat Cytosolic 5'-nucleotidase III-like protein
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Nt5c3b
• Gene Full Name: 5'-nucleotidase, cytosolic IIIB
• Alias Symbols: 5'-nucleotidase, cytosolic III-like;7-methylguanosine nucleotidase;7-methylguanosine phosphate-specific 5'-nucleotidase;cN-III-like protein;cytosolic 5'-nucleotidase 3B;cytosolic 5'-nucleotidase III-like protein;cytosolic 5-nucleotidase III-like protein-like;N(7)-methylguanylate 5'-phosphatase;Nt5c3l.
• NCBI Gene Id: 360629
• Protein Name: Q6AYP7
• Description of Target: 7-methylguanosine phosphate-specific 5'-nucleotidase
• Uniprot ID: https://www.uniprot.org/uniprot/Q6AYP7
• Protein Accession #: NP_001007724.2
• Nucleotide Accession #: NM_001007723.2
• Protein Size (# AA): 300
• Molecular Weight: 35 kDa