NR0B2 ELISA Kit (Human) (OKCD00321)

Catalog No: OKCD00321 (Formerly GWB-KBBSU8)

Size:96WELLS

Human NR0B2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Subfamily 0,Group B,Member 2 (NR0B2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Receptor Subfamily 0,Group B,Member 2 (NR0B2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Receptor Subfamily 0,Group B,Member 2 (NR0B2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nuclear Receptor Subfamily 0,Group B,Member 2 (NR0B2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Receptor Subfamily 0,Group B,Member 2 (NR0B2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Receptor Subfamily 0,Group B,Member 2 (NR0B2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NR0B2 Microplate: 96 Wells (12 x 8 Well Strips)
  • NR0B2 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated NR0B2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.054 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Small Heterodimer Partner (SHP). No significant cross-reactivity or interference between Small Heterodimer Partner (SHP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: NR0B2
• Gene Full Name: nuclear receptor subfamily 0 group B member 2
• Alias Symbols: nuclear receptor SHP;nuclear receptor subfamily 0 group B member 2;orphan nuclear receptor SHP;SHP;SHP1;small heterodimer partner.
• NCBI Gene Id: 8431
• Protein Name: Nuclear receptor subfamily 0 group B member 2
• Description of Target: Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1.
• Uniprot ID: Q15466
• Protein Accession #: NP_068804
• Nucleotide Accession #: NM_021969.2
• Protein Size (# AA): 257