Noxa Antibody (OAPB00138)

Catalog No: OAPB00138

Size:100UG

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Western blot analysis of Noxa in human stomach tissue lysate with Noxa antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.

Immunohistochemistry of Noxa in human stomach tissue with Noxa antibody at 1 μg/mL.

Immunofluorescence of Noxa in Human Stomach cells with Noxa antibody at 10 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. Noxa Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: Noxa antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Noxa antibody was raised against a synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa. The immunogen is located within the first 50 amino acids of Noxa.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-Noxa antibody is Catalog # OEPB02454
• Application Info: Noxa antibody can be used for detection of Noxa by Western blot at 0.5 to 1 ug/ml. Human stomach cell lysate can be used as a positive control. Noxa antibody is human, mouse and rat reactive. This product is for research use only. (Optimal dilution should be determined by user)
• Reference: Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000; 288:1053-8.;Nakano K and Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol. Cell 2001; 7:683-94.;Yu J, Zhang L, Hwang PM, et al. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol. Cell 2001; 7:673-82.;Eferl R, Ricci R, Kenner L, et al. Liver tumor development. c-Jun antagonizes the proapoptotic activity of p53. Cell 2003; 112:181-92.

Target Info

• Gene Symbol: Pmaip1
• Gene Full Name: phorbol-12-myristate-13-acetate-induced protein 1
• Alias Symbols: N;Noxa;phorbol-12-myristate-13-acetate-induced protein 1;protein Noxa.
• NCBI Gene Id: 58801
• Protein Name: Phorbol-12-myristate-13-acetate-induced protein 1|phorbol-12-myristate-13-acetate-induced protein 1 [Mus musculus]
• Description of Target: Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1 (By similarity). Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1.
• Uniprot ID: Q9JM54
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_067426