NOD1 Antibody (OAGA00722)

Catalog No: OAGA00722

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
OAGA00722 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NOD1(OAGA00722) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: This gene encodes a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid. Multiple alternatively spliced transcript variants differring in the 5' UTR have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Raji
  Cellular Localization: Cytoplasm
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 698 and 953 of NOD1 (Uniprot ID#Q9Y239)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: NOD1
• Gene Full Name: Nucleotide-binding oligomerization domain-containing protein 1
• Alias Symbols: CARD4, NLRC1, CLR7.1
• NCBI Gene Id: 10392
• Protein Name: Nucleotide-binding oligomerization domain-containing protein 1
• Uniprot ID: Q9Y239
• Protein Accession #: NP_006083.1
• Molecular Weight: 108 kDa