- Description of Target:
- The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency.
- Gene Symbol:
- Official Gene Full Name:
- NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
- NCBI Gene Id:
- Alias Symbols:
- CI-75Kd; MGC26839; PRO1304; CI-75k
- Tissue Tool:
- Find tissues and cell lines supported to express NDUFS1.
- Protein Accession #:
- Nucleotide Accession#:
- Swissprot Id:
- Protein Name:
- NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
- Protein Size (# AA):
- Molecular Weight:
- Partner Proteins:
- CASP3, MDH2, CASP3, TNFAIP3
- The immunogen for anti-NDUFS1 antibody: synthetic peptide directed towards the middle region of human NDUFS1
- Product Format:
- Lyophilized powder
- Affinity Purified
- Complete computational species homology data:
- NDUFS1 antibody - middle region (ARP56609_P050)
- Predicted Homology Based on Immunogen Sequence:
- Goat: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Dog: 92%; Guinea pig: 92%; Pig: 92%; Zebrafish: 78%
- Species Reactivity:
- Dog, Goat, Guinea pig, Horse, Human, Mouse, Pig, Zebrafish
- Datasheets / Downloads:
- Printable datasheet for
anti-NDUFS1 antibody- ARP56609_P050
- Peptide Sequence:
- Synthetic peptide located within the following region: TPPGLAREDWKIIRALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDIE
- Blocking Peptide:
- For anti-NDUFS1 antibody is Catalog # AAP56609 (Previous Catalog # AAPP39316)
- Reconstitution and Storage:
- Add 50 ul of distilled water. Final anti-NDUFS1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20C. Avoid repeat freeze-thaw cycles.
NDUFS1 antibody cell lysate
Department of Biochemistry
University of Utah SOM
NDUFS1 ran as expected.
Western Blot Protocol:
Cell lysates were loaded on the protein gel and separated using gel electrophoresis.
After the transfer, the nitrocellulose membrane was blocked in 5% BSA TBST for an hour at RT.
The membrane was incubated overnight at 4C in primary antibody dilution (TBST, 5%BSA with 1:1000 dilution).
The following day the membrane was washed 3X in TBST and blotted in secondary anti-rabbit antibody (TBST, 5%BSA with 1:5000 dilution) for 1 hour at RT.
The membrane was washed 3x in TBST and developed using Western Lightening Chemiluminescence reagent.
Aviva Systems Biology is the original manufacturer of this NDUFS1 antibody (ARP56609_P050)
Click here to view the NDUFS1 antibody Western Blot Protocol
Product Datasheet Link: NDUFS1 antibody (ARP56609_P050)
WB Suggested Anti-NDUFS1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Fetal Liver
Western Blot image:
Description of Target: The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency.
Questions pertaining to this data can be directed to firstname.lastname@example.org
Aviva Systems Biology’s NDUFS1 antibody (ARP56609_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at email@example.com.
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Product Review: NDUFS1 antibody-middle region (ARP56609_P050) in Human, mouse, rat liver and muscle using Western Blot
Researcher: Dr. Bruno Guigas, PhD, Dept. of Molecular Cell Biology, Leiden University Medical Center
Application: Western blotting
Species+tissue/cell type: Human, mouse, rat liver and muscle
How many ug’s of tissue/cell lysate run on the gel:
1: 30 ug Human liver
2: 30 ug Rat liver
3: 30 ug Mouse (wild-type) liver
4: 30 ug Mouse [AMPK alpha 1/2(-/-)] liver
5: 30 ug Human muscle
6: 30 ug Rat muscle
7: 30 ug Mouse muscle
Primary antibody dilution: 1:1000
Secondary antibody dilution: 1:10000
1. How do Aviva’s reagents play a role in your experimental goals?
Reliable antibodies are crucial for us.
2. How many different experimental trials were conducted using the antibody sample?
1 set of WB experiment was conducted.
3. What type of experimental sample are you using and how did you prepare it?
With have used 6 or 7 different samples (30 ug per lane):
1. Human liver
2. Rat liver
3. Wild-type mouse liver
4. AMPKa1+2-/- mouse liver
5. Human muscle
6. Rat muscle
7. Mouse muscle
A small piece (~30mg) of every organ was homogenized by Ultra-Turax (22.000 rpm; 2x5 sec) in a 6:1 (v/w) ratio of ice-cold buffer containing: 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (pH 7.6), 50 mM sodium fluoride, 50 mM potassium chloride, 5 mMsodium pyrophosphate, 1 mMethylenediaminetetraacetic acid, 1 mM ethylene glycol tetraacetic acid, 5 mM β-glycerophosphate, 1 mM sodium vanadate, 1 mMdithiothreitol, 1% nonylphenoxypolyethoxylethanol (Tergitol-type NP40) and protease inhibitors cocktail (Complete, Roche, Mijdrecht, The Netherlands). Homogenates were centrifuged (13,200 rpm; 15 min, 4°C) and the protein content of the supernatant was determined using a bicinchoninic acid (BCA) protein assay kit (BCA Protein Assay Kit, Thermo Scientific Pierce Protein Research Products, Rockford, US).
4. What applications did you test the antibody in? Please include dilutions of the primary and secondary reagents.
The antibodies were tested for Western Blot application (see above)
Dilution primary antibodies: 1/1000 for all
Dilution secondary antibodies: 1/10000 for all
5. What controls were used in your experiment? Please include your positive control.
No specific control samples but the proteins of interest are either expressed in all or in some of the samples used.
6. For IHC, what antigen retrieval method did you use?
7. How did you store the antibody after re-suspension?
All the antibodies were stored at -20C.
8. Please provide the protocol for your application procedure. Please be as detailed as possible.
Proteins (30 µg) were separated by either 12.5 or 10% SDS-PAGE followed by transfer to a polyvinylidene fluoride transfer membrane. Membranes were blocked for 1 h at room temperature in tris-buffered saline tween-20 buffer with 5% non-fat dry milk followed by an overnight incubation with antibodies. Blots were then incubated with horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature. Bands were visualized by enhanced chemiluminescence.
9. Please provide an image of your results. How does it compare to your previous images?
See the attachment image.
10. How would you rate this antibody on a scale from 1-5? Why?
5 very good (only one major specific band)
11. Would you use this antibody in future experiments?
The NDUFS1 is quite specific and would certainly be interesting for forthcoming experiments.
12. Do you believe the information about the reagent on Aviva’s website is correct?
15. If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not?