NDC80 Antibody (OAAF02003)

Catalog No: OAAF02003 (Formerly GWB-ASD277)

Size:100 ug

Western blot analysis of extracts from Jurkat cells, using KNTC2 Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HuvEc cells, using KNTC2 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human KNTC2.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: KNTC2 antibody detects endogenous levels of total KNTC2 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IF: 1:100~1:500; ELISA: 1:20000
• Reference: Chen Y., Mol. Cell. Biol. 17:6049-6056(1997).
  The MGC Project Team, Genome Res. 14:2121-2127(2004).
  Zheng L., Mol. Cell. Biol. 19:5417-5428(1999).

Target Info

• Gene Symbol: NDC80
• Gene Full Name: NDC80 kinetochore complex component
• Alias Symbols: HEC;HEC1;highly expressed in cancer protein;highly expressed in cancer, rich in leucine heptad repeats;HsHec1;hsNDC80;kinetochore associated 2;kinetochore protein Hec1;kinetochore protein NDC80 homolog;kinetochore-associated protein 2;KNTC2;NDC80 homolog, kinetochore complex component;NDC80 kinetochore complex component homolog;retinoblastoma-associated protein HEC;TID3.
• NCBI Gene Id: 10403
• Protein Name: Kinetochore protein NDC80 homolog
• Description of Target: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).
• Uniprot ID: O14777
• Protein Accession #: NP_006092.1
• Molecular Weight: 73 kDa