MIA ELISA Kit (Rat) (OKCD02642)

Catalog No: OKCD02642

Size:96WELLS

Rat MIA ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	95-105%	90-101%	95-103%	91-98%
  EDTA Plasma (n=5)	90-99%	90-97%	89-102%	78-101%
  Heparin Plasma (n=5)	83-102%	89-104%	91-105%	91-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Inhibitory Activity Protein 1 (MIA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Inhibitory Activity Protein 1 (MIA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Inhibitory Activity Protein 1 (MIA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Melanoma Inhibitory Activity Protein 1 (MIA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	84-97	88
  EDTA Plasma (n=5)	88-101	95
  Heparin Plasma(n=5)	96-103	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanoma Inhibitory Activity Protein 1 (MIA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanoma Inhibitory Activity Protein 1 (MIA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MIA Microplate: 96 Wells (12 x 8 Well strips)
  • MIA Lyophilized Standard: 2
  • 100X Biotinylated MIA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.57 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Melanoma Inhibitory Activity Protein 1 (MIA1). No significant cross-reactivity or interference between Melanoma Inhibitory Activity Protein 1 (MIA1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mia
• Gene Full Name: MIA SH3 domain containing
• Alias Symbols: cartilage-derived retinoic acid-sensitive protein;Cdrap;CD-RAP;melanoma inhibitory activity 1;melanoma inhibitory activity protein;melanoma inhibitory activity/condrocyte-derived retinoic acid sensitive protein homolog (MIA/CD-RAP);melanoma-derived growth regulatory protein;melanoma-derived growth regulatory protein-like;Mia1.
• NCBI Gene Id: 81510
• Protein Name: Melanoma-derived growth regulatory protein
• Description of Target: May function during cartilage development and maintenance.
• Uniprot ID: Q62946
• Protein Accession #: NP_110479.1
• Nucleotide Accession #: NM_030852.2
• Protein Size (# AA): 130