LPCAT1 ELISA Kit (Human) (OKCD01120)

Catalog No: OKCD01120 (Formerly GWB-KBBBH8)

Size:96WELLS

Human LPCAT1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	89-104%	91-98%	79-89%	84-98%
  EDTA plasma(n=5)	93-101%	80-94%	90-103%	80-104%
  heparin plasma(n=5)	91-104%	97-105%	97-104%	98-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysophosphatidylcholine Acyltransferase 1 (LPCAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysophosphatidylcholine Acyltransferase 1 (LPCAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysophosphatidylcholine Acyltransferase 1 (LPCAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	93-101	96
  EDTA plasma(n=5)	85-98	94
  heparin plasma(n=5)	97-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LPCAT1 Microplate: 96 Wells (12 x 8 Well Strips)
  • LPCAT1 Lyophilized Standard: 2 x 200 ng
  • 100X Biotinylated LPCAT1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 1.15 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lysophosphatidylcholine Acyltransferase 1 (LPCAT1). No significant cross-reactivity or interference between Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: LPCAT1
• Gene Full Name: lysophosphatidylcholine acyltransferase 1
• Alias Symbols: 1-acylglycerol-3-phosphate O-acyltransferase;1-acylglycerophosphocholine O-acyltransferase;1-alkenylglycerophosphocholine O-acyltransferase;1-alkylglycerophosphocholine O-acetyltransferase;acetyl-CoA:lyso-PAF acetyltransferase;acetyl-CoA:lyso-platelet-activating factor acetyltransferase;acyltransferase-like 2;AGPAT10;AGPAT9;AYTL2;LPC acyltransferase 1;lpcat;LPCAT-1;lyso-PAF acetyltransferase;lysoPAFAT;lysoPC acyltransferase 1;lysophosphatidylcholine acyltransferase 1;PFAAP3;phosphonoformate immuno-associated protein 3.
• NCBI Gene Id: 79888
• Protein Name: Lysophosphatidylcholine acyltransferase 1
• Description of Target: Possesses both acyltransferase and acetyltransferase activities (PubMed:16864775, PubMed:21498505). Activity is calcium-independent (By similarity). Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC) (PubMed:21498505). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively (PubMed:16704971). May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology (PubMed:16864775). Involved in the regulation of lipid droplet number and size (PubMed:25491198).
• Uniprot ID: Q8NF37
• Protein Accession #: NP_079106
• Nucleotide Accession #: NM_024830.3
• Protein Size (# AA): 534