LHCGR ELISA Kit (Mouse) : 96 Wells (OKEH00561)

Catalog No: OKEH00561

Size:96T

Mouse LHCGR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology LHCGR ELISA Kit (Mouse) (OKEH00561) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mouse LHCGR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Mouse LHCGR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mouse LHCGR captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: <= 6.7%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.8% (n = 20)
  Mean Inter-assay CV%: < 9.3% (n = 20)
• ELISA Kit Component:
  • LHCGR Microplate: 96 Wells (12 x 8 Well strips)
  • LHCGR Lyophilized Standard: 2
  • 100X Biotinylated LHCGR Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.09 ng/mL
• Specificity: Natural and recombinant Mouse Lutropin-choriogonadotropic hormone receptor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Lhcgr
• Gene Full Name: luteinizing hormone/choriogonadotropin receptor
• Alias Symbols: Gpcr19;Gpcr19-rs1;L;LH;LH/CG-R;Lhr;LH-R;LSH-R;Luteinizing hormone receptor;lutropin-choriogonadotropic hormone receptor.
• NCBI Gene Id: 16867
• Protein Name: P30730
• Description of Target: Lutropin-choriogonadotropic hormone receptor
• Uniprot ID: https://www.uniprot.org/uniprot/P30730
• Protein Accession #: NP_038610.1
• Nucleotide Accession #: NM_013582.2
• Protein Size (# AA): 700
• Molecular Weight: 78 kDa