LECT2 ELISA Kit (Mouse) (OKCD02685)

Catalog No: OKCD02685

Size:96WELLS

Mouse LECT2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	85-99%	90-101%	84-99%	98-105%
  EDTA Plasma (n=5)	87-101%	89-103%	83-99%	95-105%
  Heparin Plasma (n=5)	97-104%	99-105%	88-98%	79-90%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukocyte Cell Derived Chemotaxin 2 (LECT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukocyte Cell Derived Chemotaxin 2 (LECT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukocyte Cell Derived Chemotaxin 2 (LECT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leukocyte Cell Derived Chemotaxin 2 (LECT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90-103	97
  EDTA Plasma (n=5)	97-105	102
  Heparin Plasma(n=5)	93-105	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukocyte Cell Derived Chemotaxin 2 (LECT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukocyte Cell Derived Chemotaxin 2 (LECT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LECT2 Microplate: 96 Wells (12 x 8 Well strips)
  • LECT2 Lyophilized Standard: 2
  • 100X Biotinylated LECT2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 32 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Leukocyte Cell Derived Chemotaxin 2 (LECT2). No significant cross-reactivity or interference between Leukocyte Cell Derived Chemotaxin 2 (LECT2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Lect2
• Gene Full Name: leukocyte cell-derived chemotaxin 2
• Alias Symbols: chM-II;chondromodulin II;LECT-2;leukocyte cell-derived chemotaxin-2.
• NCBI Gene Id: 16841
• Protein Name: Leukocyte cell-derived chemotaxin-2
• Description of Target: Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation.
• Uniprot ID: O88803
• Protein Accession #: NP_034832.2
• Nucleotide Accession #: NM_010702.2
• Protein Size (# AA): 151