ITGAM ELISA Kit (Human) : 96 Wells (OKEH01215)

Catalog No: OKEH01215

Size:96T

Human ITGAM ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ITGAM ELISA Kit (Human) (OKEH01215) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ITGAM has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ITGAM is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ITGAM captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.1% (n = 20)
  Mean Inter-assay CV%: < 10.2% (n = 20)
• ELISA Kit Component:
  • ITGAM Microplate: 96 Wells (12 x 8 Well strips)
  • ITGAM Lyophilized Standard: 2
  • 100X Biotinylated ITGAM Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.1 ng/mL
• Specificity: Natural and recombinant Human Integrin alpha-M
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ITGAM
• Gene Full Name: integrin subunit alpha M
• Alias Symbols: antigen CD11b (p170);CD11 antigen-like family member B;CD11B;cell surface glycoprotein MAC-1 subunit alpha;complement component 3 receptor 3 subunit;CR-3 alpha chain;CR3A;integrin alpha-M;integrin, alpha M (complement component 3 receptor 3 subunit);leukocyte adhesion receptor MO1;MAC-1;MAC1A;macrophage antigen alpha polypeptide;macrophage-1 antigen alpha subunit;MO1A;Neutrophil adherence receptor;neutrophil adherence receptor alpha-M subunit;SLEB6.
• NCBI Gene Id: 3684
• Protein Name: P11215
• Description of Target: Integrin alpha-M
• Uniprot ID: https://www.uniprot.org/uniprot/P11215
• Protein Accession #: NP_000623.2
• Nucleotide Accession #: NM_000632.3
• Protein Size (# AA): 1152
• Molecular Weight: 127 kDa