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Datasheets/Manuals | Printable datasheet for IRX3 Antibody (OAAF02343) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human IRX3. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | IRX3 antibody detects endogenous levels of total IRX3 protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | IHC: 1:50~1:100; ELISA: 1:20000 |
Reference | Hansen L., Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases. The MGC Project Team, Genome Res. 14:2121-2127(2004). Lewis M.T., Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases. |
Gene Symbol | IRX3 |
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Gene Full Name | iroquois homeobox 3 |
Alias Symbols | homeodomain protein IRXB1;iroquois homeobox protein 3;iroquois-class homeodomain protein IRX-1;iroquois-class homeodomain protein IRX-3;IRX-1;IRXB1. |
NCBI Gene Id | 79191 |
Protein Name | Iroquois-class homeodomain protein IRX-3 |
Description of Target | Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells (By similarity). |
Uniprot ID | P78415 |
Protein Accession # | NP_077312.2 |
Molecular Weight | 52 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "IRX3 Antibody (OAAF02343)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "IRX3 Antibody (OAAF02343)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "IRX3 Antibody (OAAF02343)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "IRX3 Antibody (OAAF02343)"?
This target may also be called "homeodomain protein IRXB1;iroquois homeobox protein 3;iroquois-class homeodomain protein IRX-1;iroquois-class homeodomain protein IRX-3;IRX-1;IRXB1." in publications.
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What is the shipping cost for "IRX3 Antibody (OAAF02343)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "IRX3 Antibody (OAAF02343)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "IRX3 Antibody (OAAF02343)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "52 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "IRX3 Antibody (OAAF02343)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "IRX3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "IRX3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "IRX3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "IRX3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "IRX3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "IRX3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.