Catalog No: OKDD00813 (Formerly GWB-KBBMR2)
Size:96 Wells
Price: $700.00
SKU
OKDD00813
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for IL11Ra ELISA Kit (Mouse) (OKDD00813) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked immunosorbent assay | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology IL11Ra ELISA Kit (Mouse) (OKDD00813) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IL11Ra has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL11Ra is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL11Ra captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV<10% Mean Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates | ||||||||||||||||||||||
Sensitivity | <0.061ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of IL11Ra. No significant cross-reactivity or interference between IL11Ra and analogues was observed. | ||||||||||||||||||||||
Assay Info | Quantitative Sandwich ELISA |
Gene Symbol | Il11ra1 |
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Gene Full Name | interleukin 11 receptor, alpha chain 1 |
Alias Symbols | AI314697;enhancer trap locus homolog 2;etl-2;GP130;Il;Il-;IL-11 receptor subunit alpha-1;IL-11R subunit alpha-1;Il11ra;Il-11ra;IL-11RA1;Il11ra2;Il-11ra-alpha;IL-11R-alpha-1;interleukin 11 receptor, alpha chain 2;interleukin-11 receptor subunit alpha-1;locus 2;N;novel cytokine receptor 1;NR1;NR-1. |
NCBI Gene Id | 16157 |
Protein Name | Interleukin-11 receptor subunit alpha-1 |
Description of Target | Receptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. The IL11/IL11RA/IL6ST complex may be involved in the control of proliferation and/or differentiation of skeletogenic progenitor or other mesenchymal cells. Essential for the normal development of craniofacial bones and teeth. |
Uniprot ID | Q64385 |
Protein Accession # | NP_001156873.1 |
Nucleotide Accession # | NM_001163401.1 |
Protein Size (# AA) | 432 |
Molecular Weight | 47 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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