IL-1RA/IL1RN ELISA Kit (Mouse) (OKBB00437)

Catalog No: OKBB00437

Size:96 Wells

Mouse IL-1RA/IL1RN ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse IL-1RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-1RA has been precoated onto 96-well plates. Standards(E.coli, R27-Q178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IL-1RA amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	476	1326	2760	595	1533	3185
  SD	30	72.93	129.72	46.41	95	172
  CV%	6.3	5.5	4.7	7.8	6.2	5.4

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse IL-1RA Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse IL-1RA Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse IL-1RA Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse IL-1RA
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: R27-Q178
• Description: For quantitative detection of mouse IL-1RA in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: IL1RN
• Gene Full Name: interleukin 1 receptor antagonist
• Alias Symbols: ICIL 1RA, IL1 inhibitor, ICIL-1RA, ICIL1RA, IL-1ra, IL-1RN, IL1 inhibitor, IL1F3, IL1RA_HUMAN, IL1RN, Interleukin 1 Receptor Antagonist Protein, IRAP
• NCBI Gene Id: 16181
• Protein Name: Interleukin-1 receptor antagonist protein
• Description of Target: The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization. IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.
• Uniprot ID: P25085
• Protein Accession #: NP_001034790.1
• Nucleotide Accession #: NM_001039701.3