HK2 ELISA Kit (Human) (OKCD00731)

Catalog No: OKCD00731 (Formerly GWB-KBBZO4)

Size:96WELLS

Human HK2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	98-105%	92-101%	81-95%	95-105%
  EDTA plasma(n=5)	91-99%	81-98%	83-93%	93-101%
  heparin plasma(n=5)	89-96%	91-98%	80-102%	94-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hexokinase 2 (HK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hexokinase 2 (HK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hexokinase 2 (HK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hexokinase 2 (HK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	81-98	94
  EDTA plasma(n=5)	82-98	89
  heparin plasma(n=5)	78-103	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hexokinase 2 (HK2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hexokinase 2 (HK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HK2 Microplate: 96 Wells (12 x 8 Well Strips)
  • HK2 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated HK2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 13.1 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hexokinase 2 (HK2). No significant cross-reactivity or interference between Hexokinase 2 (HK2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HK2
• Gene Full Name: hexokinase 2
• Alias Symbols: hexokinase type II;hexokinase-2;hexokinase-2, muscle;hexokinase-B;HKII;HXK2;muscle form hexokinase.
• NCBI Gene Id: 3099
• Protein Name: Hexokinase-2
• Description of Target: Hexokinase-2
• Uniprot ID: P52789
• Protein Accession #: NP_000180
• Nucleotide Accession #: NM_000189.4
• Protein Size (# AA): 917