Goat Anti-GIPC1 / NIP Antibody (OAEB01178)

Catalog No: OAEB01178 (Formerly GWB-341111)

Size:100UG

HEK293 overexpressing GIPC1 isoform 1 (RC216466) and probed with OAEB01178 (mock transfection in first lane), tested by Origene.

OAEB01178 staining (0.03ug/ml) of human HeLa cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: GIPC1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 90%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%
• Peptide Sequence: C-GAIGDAKVGRY
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:128000.
  Western Blot: Approx 38kDa and strong 23-24kDa bands observed in lysates of cell line HeLa, and approx 24kDa also observed in Human Pancreas, Kidney and Muscle lysates (calculated MW of 36.0kDa according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1kDa according to NP_974198.1 and NP_974223.1). In transfected HEK293 transiently expressing GIPC1 isoform 1 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml.
• Reference: * Valdembri D, Caswell PT, Anderson KI, Schwarz JP, Konig I, Astanina E, Caccavari F, Norman JC, Humphries MJ, Bussolino F, Serini G.; Neuropilin-1/GIPC1signaling regulates alpha5beta1 integrin traffic and function in endothelial cells.; PLoS Biol. 2009 Jan 27;7(1):e25.; Pubmed 19175293
  * Zerbinatti CV, Cordy JM, Chen CD, Guillily M, Suon S, Ray WJ, Seabrook GR, Abraham CR, Wolozin B.; Oxysterol-binding protein-1 (OSBP1) modulates processing and trafficking of the amyloid precursor protein.; Mol Neurodegener. 2008 Mar 18;3:5.; Pubmed 18348724
  * De Vries L, Lou X, Zhao G, Zheng B, Farquhar MG.; GIPC, a PDZ domain containing protein, interacts specifically with the C terminus of RGS-GAIP.; Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12340-5.; Pubmed 9770488

Target Info

• Gene Symbol: Gipc1|GIPC1
• Gene Full Name: GIPC PDZ domain containing family member 1|GIPC PDZ domain containing family, member 1
• Alias Symbols: C19orf3;GAIP C-terminus-interacting protein;GIPC;Glut1;GLUT1 C-terminal binding protein;GLUT1 C-terminal-binding protein;GLUT1CBP;Glut1CIP;Hs.6454;IGF-1 receptor interacting protein 1;IIP-1;neurophilin1-IP;NIP;OPDM2;PDZ domain-containing protein GIPC1;regulator of G-protein signaling 19 interacting protein 1;regulator of G-protein signalling 19 interacting protein 1;Rgs1;Rgs19;RGS19-interacting protein 1;RGS19IP1;RGS-GAIP interacting protein GIPC;RGS-GAIP interacting protein, C-terminus;RGS-GAIP-interacting protein;semaF cytoplasmic domain associated protein;semaF cytoplasmic domain-associated protein 1;Semc;SEMCAP;Semcap1;SEMCAP-1;syne;SYNECTIIN;SYNECTIN;Tax;Tax interacting protein, clone 2;tax interaction protein 2;TaxIP2;TIP;TIP-2.
• NCBI Gene Id: 10755|67903|83823
• Protein Name: PDZ domain-containing protein GIPC1
• Uniprot ID: O14908
• Protein Accession #: NP_005707.1
• Molecular Weight: 36.0; 26.1