Goat Anti-Clik1 / STK35 Antibody (OAEB02379)

Catalog No: OAEB02379 (Formerly GWB-14F8A0)

Size:100UG

OAEB02379 (1ug/ml) staining of human testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02379 (10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: STK35
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 84%
• Peptide Sequence: ELETRMDQVTCAA
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Approx 45kDa band observed in human testis lysates (calculated MW of 44.6kDa according to NP_543026. Recommended concentration: 1-3ug/ml.
  Immunohistochemistry: In paraffin embedded human testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration, 10ug/ml.
• Reference: * Goyal P, Behring A, Kumar A, Siess W.; Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates.; PLoS One. 2009 Sep 16;4(9):e6981.; Pubmed 19756140
  * Vallenius T, Makela TP.; Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein.; J Cell Sci. 2002 May 15;115(Pt 10):2067-73.; Pubmed 11973348

Target Info

• Gene Symbol: Stk35|STK35
• Gene Full Name: serine/threonine kinase 35
• Alias Symbols: 1700054C12Rik;CLI;CLIK1;CLIK-1;CLP-36 interacting kinase;CLP-36-interacting kinase 1;PDLIM1-interacting kinase 1;serine threonine kinase 35 long form;serine/threonine-protein kinase 35;serine/threonine-protein kinase 35 L1;Stk35l1.
• NCBI Gene Id: 140901|67333
• Protein Name: Serine/threonine-protein kinase 35
• Uniprot ID: Q8TDR2
• Protein Accession #: NP_543026.2
• Molecular Weight: 58.1