Goat Anti-ALS2CR2 / ILPIP Antibody (OAEB02406)

Catalog No: OAEB02406 (Formerly GWB-D05922)

Size:100UG

OAEB02406 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing ILPIP (RC203432) and probed with OAEB02406 (mock transfection in first lane), tested by Origene.

OAEB02406 (3.8ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Additional Information: Reactivity Info: Tested: Human; Expected: Human, Dog, Cow
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: STRADB
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 92%; Human: 100%; Pig: 84%; Rabbit: 84%
• Peptide Sequence: CDFPDEKDSYWEF
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Approx 35kDa band observed in Human Heart, Liver and Muscle lysates (calculated MW of 47.0kDa according to NP_061041.2 (alpha isoform) and 31.2kDa according to AAF71042.1(beta isoform). In transfected HEK293 transiently expressing ILPIP a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration 1-3ug/ml.
  Immunohistochemistry: In paraffin embedded Human Heart shows staining of myocardial fibres in transverse section. Recommended concentration, 3-5ug/ml.
• Reference: * Sanna MG, da Silva Correia J, Luo Y, Chuang B, Paulson LM, Nguyen B, Deveraux QL, Ulevitch RJ.; ILPIP, a novel anti-apoptotic protein that enhances XIAP-mediated activation of JNK1 and protection against apoptosis.; J Biol Chem. 2002 Aug 23;277(34):30454-62.; Pubmed 12048196

Target Info

• Gene Symbol: STRADB
• Gene Full Name: STE20 related adaptor beta
• Alias Symbols: ALS2CR2;amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2;amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein;CALS-21;ILP-interacting protein ILPIPA;ILPIP;ILPIPA;PAPK;PRO1038;pseudokinase ALS2CR2;STE20-related kinase adapter protein beta;STE20-related kinase adaptor beta;STRAD beta.
• NCBI Gene Id: 55437
• Protein Name: STE20-related kinase adapter protein beta
• Uniprot ID: Q9C0K7
• Protein Accession #: NP_061041.2
• Molecular Weight: 47.0, 31.2