GKN1 ELISA Kit (Rat) (OKCD00332)

Catalog No: OKCD00332 (Formerly GWB-KBBBG7)

Size:96WELLS

Rat Gkn1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	82-99%	90-103%	95-102%	90-97%
  EDTA plasma(n=5)	85-97%	94-101%	98-105%	98-105%
  heparin plasma(n=5)	83-92%	78-99%	80-101%	88-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gastrokine 1 (GKN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gastrokine 1 (GKN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gastrokine 1 (GKN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Gastrokine 1 (GKN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	84-103	91
  EDTA plasma(n=5)	81-103	88
  heparin plasma(n=5)	83-92	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gastrokine 1 (GKN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gastrokine 1 (GKN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Gkn1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Gkn1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Gkn1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 0.069 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Gastrokine 1 (GKN1). No significant cross-reactivity or interference between Gastrokine 1 (GKN1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Gkn1
• Gene Full Name: gastrokine 1
• Alias Symbols: Amp18;Ca11;Fov;foveolin;gastrokine-1.
• NCBI Gene Id: 297418
• Protein Name: Foveolin
• Description of Target: Foveolin
• Uniprot ID: Q6SJV7
• Protein Accession #: NP_945323
• Nucleotide Accession #: NM_198972.1
• Protein Size (# AA): 183