ERBB3 ELISA Kit (Mouse) (OKCD02526)

Catalog No: OKCD02526

Size:96WELLS

Mouse ERBB3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-95%	89-96%	94-103%	81-105%
  EDTA Plasma (n=5)	93-101%	86-97%	80-95%	99-105%
  Heparin Plasma (n=5)	83-95%	79-101%	84-102%	83-93%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-96	87
  EDTA Plasma (n=5)	82-102	92
  Heparin Plasma(n=5)	91-101	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ERBB3 Microplate: 96 Wells (12 x 8 Well strips)
  • ERBB3 Lyophilized Standard: 2
  • 100X Biotinylated ERBB3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 3.4 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3). No significant cross-reactivity or interference between V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Erbb3
• Gene Full Name: erb-b2 receptor tyrosine kinase 3
• Alias Symbols: avian erythroblastosis oncogene B 3 receptor;C76256;c-erbB-3;Erbb;Erbb-;Erbb-3;Erbb3r;glial growth factor receptor;HER;Her3;proto-oncogene-like protein c-ErbB-3;receptor tyrosine-protein kinase erbB-3;v-erb-b2 erythroblastic leukemia viral oncogene homolog 3.
• NCBI Gene Id: 13867
• Protein Name: Receptor tyrosine-protein kinase erbB-3
• Description of Target: Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. May also be activated by CSPG5.
• Uniprot ID: Q61526
• Protein Accession #: NP_034283.1
• Nucleotide Accession #: NM_010153.1
• Protein Size (# AA): 1339